Figure 1.

Ca²⁺ mobilizers induce adhesion of T cell LFA-1 to ICAM-1Fc. T cells were treated with ionomycin, thapsigargin, and dBHQ at the indicated concentrations and incubated on ICAM-1Fc–coated 96-well plates for 30 min at 37°C. The proportion of adhering cells was calculated as a percentage of total cells added per well. For all stimulants, adhesion was reduced to background levels by means of anti–LFA-1 mAb used at 10 μg/ml. An experiment representative of five similar experiments is presented.