Table I.

Quantitation of the Intracellular Distribution of Wild-type and Mutant ARF6 Proteins

Cells transfected with		Plasma membrane/ invaginations		Pericentriolar vesicles and tubules
ARF6 (low expression)		4		38
ARF6 (high expression)		125		66
ARF6(Q67L)		159		34
ARF6(T27N)		18		163

CHO cells on monolayers expressing ARF6 wild-type or mutant proteins were fixed and processed for cryoimmunogold labeling and labeled waith anti-ARF6 antisera followed by protein A–10-nm gold. The data represents an average of 10-nm gold particle count obtained from 25 cells.