Table I.
Apparent Plasma Membrane Diffusion Coefficients and Dissociation Times of Cys1–GFP
| Substance added | Halftime of recovery | Apparent lateral diffusion coefficient | Apparent dissociation time constant | |||
|---|---|---|---|---|---|---|
| s | μm2/s | s | ||||
| PC-PLC (n = 15) | 0.79 ± 0.02 | 1.19 ± 0.19 | 3.5 ± 0.5 | |||
| DiC8 (n = 18) | 1.3 ± 0.1 | 0.97 ± 0.14 | 8.0 ± 1.6 | |||
| PDBu (n = 10) | 6.4 ± 0.9 | 0.26 ± 0.01 | 35.9 ± 0.8 | |||
| PMA (n = 17) | 9.8 ± 0.6 | 0.15 ± 0.04 | 98.6 ± 19.0 |
Plasma membrane photobleaching recovery measurements of Cys1-GFP. The relative halftimes of recovery were compared after addition of different diacylglycerol analogues that induced plasma membrane translocation of Cys1–GFP. Apparent diffusion coefficients and dissociation time constants were calculated as described in Materials and Methods.