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. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Eur J Cell Biol. 2007 Jun;86(6):303–313. doi: 10.1016/j.ejcb.2007.03.008

Fig. 1.

Fig. 1

Analysis of pCD secretion by HaCaT and ZR-75-1 cells. HaCaT and ZR-75-1 cells were seeded in normal growth medium followed by replacing to serum-free media for additional 48 h. Conditioned media were collected and concentrated (10×). The cell pellets were lysed in lysis buffer. Protein contents of conditioned media and lysates were determined and equal amounts of total protein were analyzed by SDS-PAGE followed by immunoblotting. pCD protein in conditioned media was detected using anti-AP antibody (A); pCD and CD levels were detected in cell lysates using anti-CD antibody (B).