Figure 1.

NAD⁺ is required for the BFA-induced redistribution of Golgi markers in permeabilized cells. Intact RBL cells (a and b) were treated with 3 μg/ml BFA for 15 min (b), or were permeabilized with 3 U/ml SLO (c–f), and incubated for 20 min at 37°C as described in Materials and Methods in the absence (c) or the presence of 150 μM NAD⁺ (e), or of 10 μg/ml BFA alone (d), or of BFA plus 150 μM NAD⁺ (f). Cells were then stained with an anti-Man II antibody. Similar results were obtained using the helix pomatia lectin, a marker of the cis Golgi compartment (not shown). Experiments were repeated four times in duplicate with similar results. Bar, 5 μm