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. 1997 Dec 1;139(5):1109–1118. doi: 10.1083/jcb.139.5.1109

Figure 2.

Figure 2

Ultrastructure of the Golgi complex in permeabilized cells: NAD+ is required for the effect of BFA. Intact RBL cells (a and b) were treated with 3 μg/ml BFA for 15 min (b), or were permeabilized with 3 U/ml SLO (c–f), and incubated for 20 min at 37°C in the absence (c) or in the presence of BFA alone (d), or of 150 μM NAD+ (e), or of BFA in combination with 150 μM NAD+ (f). The cells were then processed for electron microscopy. Experiments were repeated at least three times in duplicate with similar results. Bar, 0.5 μm.