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. 1997 Dec 1;139(5):1109–1118. doi: 10.1083/jcb.139.5.1109

Figure 4.

Figure 4

Pre–ADP-ribosylated cytosol replaces NAD+ in sustaining the Golgi-disassembling activity of BFA. Effects of BARS-50–containing extracts. RBL cells were permeabilized with 3 U/ml SLO and incubated with control (a–c) or ADP-ribosylated (d–i) cytosol (1 mg/ml) for 20 min at 37°C in the absence (a and d) or in the presence (b, c, and e–i) of 10 μg/ml BFA. Native BARS-50 (an extract prepared as described in Materials and Methods and diluted 10-fold in ADP-ribosylated cytosol) was added in i (with NAD+) and in g without NAD+. ADP-ribosylated BARS-50 (an extract identical to that containing native BARS-50 but prepared from ADP-ribosylated cytosol; see Materials and Methods) was added to h. Cells were fixed and labeled with anti-Man II antibody. Similar results were obtained in four different experiments. Bar, 5 μm.