Figure 7.

Pre–ADP-ribosylated cytosol prevents and native BARS-50 rescues the anti-BFA effects of dicumarol on the Golgi complex in permeabilized cells. RBL cells were permeabilized with 3 U/ml SLO, and incubated for 20 min at 37°C in a medium containing BFA (10 μg/ml) and NAD⁺ (150 μM) without (a, c, e, and g) or with 200 μM dicumarol (b, d, f, and h) in the presence of control (a and b) or pre–ADP-ribosylated (c–h) cytosol (1 mg/ml). A native BARS-50–enriched extract (see Fig. 4 legend) was added to (e) and (f), whereas ADP-ribosylated BARS-50 was added to (g) and (h). The cells were fixed and labeled with anti-Man II antibody. Similar results were obtained in three independent experiments. Bar, 5 μm.