Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Dec 1;139(5):1255–1269. doi: 10.1083/jcb.139.5.1255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Neutrophil plasma membranes are highly enriched in p205. Fractions highly enriched in plasma membranes (A) or secretory vesicles (B) were analyzed for the presence of p205 by F-actin blot overlay (C). Aliquots (100 μg protein) were fractionated on a 5% SDS–polyacrylamide gel, transferred to nitrocellulose, and then probed with ¹²⁵I-labeled F-actin. Lane 1, plasma membranes; lane 2, secretory vesicles; and lane 3, cytosol. E/M, the position of ezrin and moesin. Other cytosolic actin-binding proteins also are detected by this method (lane 3).

Neutrophil plasma membranes are highly enriched in p205. Fractions highly enriched in plasma membranes (A) or secretory vesicles (B) were analyzed for the presence of p205 by F-actin blot overlay (C). Aliquots (100 μg protein) were fractionated on a 5% SDS–polyacrylamide gel, transferred to nitrocellulose, and then probed with ¹²⁵I-labeled F-actin. Lane 1, plasma membranes; lane 2, secretory vesicles; and lane 3, cytosol. E/M, the position of ezrin and moesin. Other cytosolic actin-binding proteins also are detected by this method (lane 3).