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. 1997 Dec 1;139(5):1209–1217. doi: 10.1083/jcb.139.5.1209

Figure 3.

Figure 3

[Ca2+]i in cells expressing the chimeric FcγRIIIB/CD7. J2/3 cells (top), J2/3-CD7 cells (middle), or PMN (bottom) were preloaded with Fura 2-AM. J2/3 and J2/3-CD7 cells were then incubated for 30 min with the mAb IV.3 (anti-FcγRII), mAb 3G8 (anti-FcγRIII), mAb MEM-43 (anti-CD59), or combinations of these mAbs. PMN were incubated with mAb IV.3 F(ab), mAb 10G10 F(ab′)2 (anti-CD59), or combinations of these mAbs. Experiments were performed as described in Fig. 2. Each curve is representative of at least three independent experiments. For PMN, the change in [Ca2+]i at 140 s after the addition of crosslinking antibody was calculated and results are shown as the mean ± SEM for three independent experiments (bottom).