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. 1997 Dec 1;139(5):1209–1217. doi: 10.1083/jcb.139.5.1209

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The synergistic rise in [Ca²⁺]_i requires the influx of extracellular calcium. Changes in Fura 2-AM fluorescence after receptor crosslinking in J2/3 cells was measured as in Fig. 2 in the absence or presence of 2 mM EGTA to prevent calcium influx from the medium. (A) 2 mM EGTA was added 280 s after crosslinking. (B) 2 mM EGTA was added immediately before receptor crosslinking. Also shown is no added EGTA. (C) 2 mM EGTA was added at 0 or 300 s.