Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Dec 1;139(5):1349–1360. doi: 10.1083/jcb.139.5.1349

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Neutrophils migrate away from one chemoattractant source towards another. (a) Photographs of stained neutrophils after 2-h migration towards a distant source of LTB4 or IL-8 (1 pmol), in the presence or absence of an inverse gradient generated by LTB4 or IL-8 (10 pmol). Cells placed with one agonist migrate towards the other agonist almost as well as control cells, but do not migrate well towards a distant source of the same agonist. (b) Dose-response curves illustrate that over a wide range of concentrations of both agonists, cells can migrate away from an IL-8 source in response to a target LTB4 gradient; however, cells do not migrate away from a well containing IL-8 (⩾1 pmol) towards a distant IL-8 source. (c) Conversely, over a wide concentration range of close and distant agonists, neutrophils migrate away from an LTB4 source towards IL-8, but not towards LTB4. Each graph in b and c shows the data from a representative experiment of at least two to five performed with similar results. Error bars in b and c indicate the standard deviation of the distance migrated for four replicates.

Neutrophils migrate away from one chemoattractant source towards another. (a) Photographs of stained neutrophils after 2-h migration towards a distant source of LTB4 or IL-8 (1 pmol), in the presence or absence of an inverse gradient generated by LTB4 or IL-8 (10 pmol). Cells placed with one agonist migrate towards the other agonist almost as well as control cells, but do not migrate well towards a distant source of the same agonist. (b) Dose-response curves illustrate that over a wide range of concentrations of both agonists, cells can migrate away from an IL-8 source in response to a target LTB4 gradient; however, cells do not migrate away from a well containing IL-8 (⩾1 pmol) towards a distant IL-8 source. (c) Conversely, over a wide concentration range of close and distant agonists, neutrophils migrate away from an LTB4 source towards IL-8, but not towards LTB4. Each graph in b and c shows the data from a representative experiment of at least two to five performed with similar results. Error bars in b and c indicate the standard deviation of the distance migrated for four replicates.