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. 1997 Dec 1;139(5):1349–1360. doi: 10.1083/jcb.139.5.1349

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Gradients formed by IL-8 and LTB4 and calculated agonist concentrations for 1 pmol added in well 1. Each curve shows a representative of at least three determinations of the IL-8 (a) or LTB4 (b) concentration gradients in agarose, 30–120 min after addition of radioactive agonist, using the protocol outlined in the Materials and Methods section. Replicate IL-8 gradient measurements were very consistent in shape and magnitude, whereas LTB4 gradient measurements were more variable, especially in magnitude. The concentrations indicated were calculated for a gradient generated by 1 pmol of agonist. (c) Two replicate determinations of the LTB4 gradient at 120 min.