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. 1997 Dec 1;139(5):1337–1348. doi: 10.1083/jcb.139.5.1337

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The role of the cytoplasmic domain in the effect of Ep-CAM on cadherins. (A) The structural maps of the wild-type (wild-type) and mutant (Mu1) Ep-CAM molecules. The leader peptide (L), EGF-like domains (EGF-I, EGF-II) cysteine-poor region (CPR), the transmembrane domain (TM), and cytoplasmic domain (CYT) are marked. The cytoplasmic domain is deleted in the Mu1 molecule. (B) HCA cells were transfected with either blank vector (HMC), or the wild-type Ep-CAM (Wt), or Mu1, under the control of a metallothionein promotor. Where indicated (+Cd), the cells were cultured in the presence of 50 μm CdCl₂ for 24h before lysis. Aliquots of total cell lysates equalized by protein were probed in immunoblotting using the Ep-CAM–specific mAB. Content of N-cadherin in whole cell lysates and after the extraction of cells with 0.5% Triton X-100 was analyzed with anti-pan cadherin mAb CH-19. Note that expression of Wt Ep-CAM, but not of an approximately equal level of Mu1, affects the detergent solubility of cadherins.

The role of the cytoplasmic domain in the effect of Ep-CAM on cadherins. (A) The structural maps of the wild-type (wild-type) and mutant (Mu1) Ep-CAM molecules. The leader peptide (L), EGF-like domains (EGF-I, EGF-II) cysteine-poor region (CPR), the transmembrane domain (TM), and cytoplasmic domain (CYT) are marked. The cytoplasmic domain is deleted in the Mu1 molecule. (B) HCA cells were transfected with either blank vector (HMC), or the wild-type Ep-CAM (Wt), or Mu1, under the control of a metallothionein promotor. Where indicated (+Cd), the cells were cultured in the presence of 50 μm CdCl₂ for 24h before lysis. Aliquots of total cell lysates equalized by protein were probed in immunoblotting using the Ep-CAM–specific mAB. Content of N-cadherin in whole cell lysates and after the extraction of cells with 0.5% Triton X-100 was analyzed with anti-pan cadherin mAb CH-19. Note that expression of Wt Ep-CAM, but not of an approximately equal level of Mu1, affects the detergent solubility of cadherins.