Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Dec 1;139(5):1337–1348. doi: 10.1083/jcb.139.5.1337

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Effect of increasing expression of Ep-CAM on cell–cell interactions in L cell E-cadherin transfectants (LEC). LEC cells were supertransfected with Ep-CAM cDNA under the control of the metallothionein promotor (clones LEC-MEp.2 and LEC-MEp.6), or with blank vector (LEC-MC). Induction of Ep-CAM expression with CdCl₂ for 24 h resulted in an increased total Ep-CAM (A, immunoblot of total cell lysates) and in an increased presence of Ep-CAM molecules at the cell surface (B, flow cytometry with anti–Ep-CAM F(ab)-FLUOS conjugate). Cells detached with TC treatment were allowed to aggregate in suspension for 30 min in the presence of Ca²⁺, and the degree of cell aggregation was determined (C). The statistical significance of the observed differences in aggregation rates was determined using the Student's t-test (p). Where indicated, cells were cultured for 24 h before the assay in the presence of CdCl₂ in culture medium. (D) The morphology of aggregates formed in 30 min by LEC-MC and LEC-MEp.6 cells (the latter noninduced and induced with 10 μM CdCl₂).

Effect of increasing expression of Ep-CAM on cell–cell interactions in L cell E-cadherin transfectants (LEC). LEC cells were supertransfected with Ep-CAM cDNA under the control of the metallothionein promotor (clones LEC-MEp.2 and LEC-MEp.6), or with blank vector (LEC-MC). Induction of Ep-CAM expression with CdCl₂ for 24 h resulted in an increased total Ep-CAM (A, immunoblot of total cell lysates) and in an increased presence of Ep-CAM molecules at the cell surface (B, flow cytometry with anti–Ep-CAM F(ab)-FLUOS conjugate). Cells detached with TC treatment were allowed to aggregate in suspension for 30 min in the presence of Ca²⁺, and the degree of cell aggregation was determined (C). The statistical significance of the observed differences in aggregation rates was determined using the Student's t-test (p). Where indicated, cells were cultured for 24 h before the assay in the presence of CdCl₂ in culture medium. (D) The morphology of aggregates formed in 30 min by LEC-MC and LEC-MEp.6 cells (the latter noninduced and induced with 10 μM CdCl₂).