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. 1997 Dec 1;139(5):1337–1348. doi: 10.1083/jcb.139.5.1337

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Expression of E-cadherin in blank vector (−MC) and Ep-CAM transfectants (clones −MEp.2 and −MEp.6) of LEC cells. The cells were cultured for 24 h in the presence or absence of 10 μM CdCl₂ in culture medium. Cells were either (A) detached from the substrate with EDTA and analyzed in flow cytometry after staining with mAb ECCD-2 to murine E-cadherin, or (B) lysed with 1% SDS, and E-cadherin was detected in total cell lysates (5 μg protein/sample) by immunoblotting with mAb 36.

Expression of E-cadherin in blank vector (−MC) and Ep-CAM transfectants (clones −MEp.2 and −MEp.6) of LEC cells. The cells were cultured for 24 h in the presence or absence of 10 μM CdCl₂ in culture medium. Cells were either (A) detached from the substrate with EDTA and analyzed in flow cytometry after staining with mAb ECCD-2 to murine E-cadherin, or (B) lysed with 1% SDS, and E-cadherin was detected in total cell lysates (5 μg protein/sample) by immunoblotting with mAb 36.