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. 1997 Dec 1;139(5):1137–1155. doi: 10.1083/jcb.139.5.1137

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Tubulation and explosive disassembly of the Golgi complex in BFA-treated cells. GFP-GalTase– expressing HeLa cells treated with BFA were imaged at 4-s time intervals using a cooled CCD microscope system at 37°C. Images shown begin after 4 min (4:00) of BFA treatment and extend until 8 min 57 s (8:57). Tubule formation was accentuated in these cells without tubule detachment. GFP-GalTase rapidly emptied from the Golgi tubular system into the ER over a period of about 14 s beginning at 8 min 33 s (8:33). Thereafter, the GFP label remained dispersed in ER membranes leaving no identifiable Golgi structures behind. Bar, 5 μm. See Quicktime movie sequence at http://dir.nichd.nih.gov/CBMB/pb4labob.htm.