Table I.
Properties of Golgi Tubules in HeLa Cells Expressing GFP-KDELR
| Untreated cells | BFA-treated cells | |||
|---|---|---|---|---|
| Length | 4.3 ± 1.7 μm (n = 16) | 4–20 μm (n = 15) | ||
| Velocity of extension | 0.6 ± 0.2 μm/s (n = 16) | 0.6 ± 0.2 μm/s (n = 15) | ||
| Range of duration | 9–120 s (n = 16) | 3–9 min (n = 15) | ||
| Tubule number | 0–8 (n = 7) | 20–30 (n = 5) | ||
| in 1-min interval |
Golgi tubules extending from the rims of Golgi elements were analyzed in untreated cells or in cells treated with BFA (2 μg/ml) at 37°C from digitized images collected on a confocal microscope. n, number of cells observed.