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. 1997 Dec 1;139(5):1281–1292. doi: 10.1083/jcb.139.5.1281

Figure 4.

Figure 4

Effect on cell viability of transient expression of wild-type and COOH-terminal truncated forms of Bcl-2 family members, with and without GFP fused at the NH2-terminus. Cells were either singly transfected with the GFP fusion constructs or cotransfected with GFP and various Bcl-2 family members. At 48 h after transfection, cells were treated with STS. The number of GFP-positive cells was counted within a given field at regular intervals, starting at the time of STS addition (∼500 GFP-positive cells at time zero was typical for each experiment). Changes in cell viability are displayed as number of glowing cells within a field and expressed as a percentage of time zero value for that field. (A) Bcl-2 constructs expressed in L929 cells. (n = 4). pcDNA3 vector control (⋄), Bcl-2 (○), GFP–Bcl-2 (•), Bcl-2 ΔCT (□), GFP–Bcl-2 ΔCT (▪), and Bax (▵) (B) Bcl-XL constructs expressed in L929 cells. (n = 4). pcDNA3 vector control (⋄), Bcl-XL (○), GFP–Bcl-XL (•), Bcl-XL ΔCT (□), GFP–Bcl-XL ΔCT (▪), and Bax (▵) (n = 4). (C) Bax constructs expressed in Cos-7 cells. (n = 3). pcDNA3 vector control (⋄), Bax (○), GFP–Bax (•), Bax ΔCT (▵), GFP–Bax ΔCT (▴).