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. Author manuscript; available in PMC: 2007 Dec 17.
Published in final edited form as: J Biol Chem. 2003 Mar 20;278(21):18997–19007. doi: 10.1074/jbc.M301800200

Fig.9. Down-regulation of Tra2β by RNAi decreases the inclusion of exon 10 in tau minigene.

Fig.9

RNAi oligonucleotide specific for human Tra2β (lane 1) or SRp40 (lane 2) or control oligonucleotide (lane 3) was cotransfected with tau minigene TauEx9−11d5(N279K) into HEK293 cells. A shows the specific down-regulation of Tra2β expression in the RNAi oligonucleotide-treated cells. The total RNAs of HEK293 cells were prepared 48 h after transfection. Tra2β-specific primers were used for RT-PCR to detect the RNA level of Tra2β. B, Western blotting results using specific anti-Tra2β in the corresponding RNAi oligonucleotide-treated cells. C, tau exon 10 splicing pattern as detected by RT-PCR. RNA amounts were comparable as shown with the internal γ actin control as shown in the lower portion of C. The ratio of exon 10+/exon 10− splicing products was decreased by Tra2β-specific RNA interference. Quantification of the results derived from three independent experiments is shown in D.