Table 1.

RNA in situ hybridization patterns of genes downregulated in GMR>sSpi vs. GMR>Ras^V12

Gene	SLR	Expression pattern	Function/homology	Reference
Disc Proper (27)
SoxN	−5.65	Differentiating photoreceptors posterior to MF	Nervous system development	(Cremazy et al., 2001)
lz	−4.06	Subset of cells posterior to the morphogenetic MF	Eye cell fate specification and differentiation	(Flores et al., 1998)
Spn43A	−3.67	Cells anterior to the MF and in posterior inter-ommatidial cells	Serine-type endopeptidase inhibitor activity	(Green et al., 2000)
CG15630	−3.49	Subgroups of cells in 3/4 columns posterior to MF (Figure 2A)	Cell adhesion
SP1029	−3.45	Band anterior to the MF; inner tarsal ring of leg imaginal disc (Figure 2B)	Proteolysis
CG31676	−3.22	Retinal basal glial cells (Figure 2F)	-
Fas2	−2.91	Differentiating photoreceptors	Cell adhesion; axonal fasciculation; synaptic plasticity	(Pignoni et al., 1997)
B-H1	−2.87	Subset of photoreceptors and accessory cells	Photoreceptors R1 and R6, pigment and lens cell differentiation	(Higashijima et al., 1992)
thisbe	−2.81	Individual differentiating cells posterior to MF	FGFR ligand. FGFR signaling in mesoderm, hindgut and heart
CG31291	−2.71	Ubiquitous	-
Pde8	−2.37	Band just anterior to MF; PE; plasmatocytes; lumen and nuclei of salivary glands. Nuclei of fat body	Signal transduction; cyclic nucleotide metabolism; mesoderm dev.
Klg	−2.14	Three rows of cells ahead of MF; ommatidial cluster; refines to R7	R7 photoreceptor cell fate commitment and differentiation	(Butler et al., 1997)
CG13966	−2.03	Band anterior to MF (Figure 2C)	-
ato	−1.95	Stripe anterior to MF, refines to R8 cells only	R8 cell specification.	(Jarman et al., 1994)
GM130	−1.92	Anterior to MF (Figure 2D)	Golgi organization and biogenesis; mitosis; protein targeting
trn	−1.91	Band of cells just anterior to MF refining to clusters of cells and a subset of differentiating cells.	Target of Egfr/PntP1 signaling in embryonic ventral ectoderm	(Chang et al., 1993)
Obp44a	−1.84	Photoreceptor axons as exiting optic stalk and optic lobe	-
CG8502	−1.71	Individual cells near posterior edge of MF for 2/3 columns; eye, leg and wing PE; plasmatocytes (Figure 2E)	Structural constituent of larval cuticle
CG5653	−1.71	Ubiquitous	-
CG15911	−1.69	Anterior D/V margins of eye disc;plasmatocytes	-
E(spl) m2	−1.68	Clusters of cells in the MF region	Notch signaling pathway	(Lai et al., 2000)
CAP	−1.64	Expressed within MF	MAPKKK cascade; cytoskeleton organization and biogenesis
CG5929	−1.61	Broad band anterior to MF	Nucleic acid metabolism; RNA-dependent transcription
Ppn	−1.58	Diffusely expressed in eye imaginal disc	Component of the ECM; metallopeptidase activity	(Kramerova et al., 2000)
Traf1	−1.56	SMW cells and clusters within MF; ZNP cells in the wing	In vivo regulator of JNK pathway	(Priess and Hirsch, 1986)
Brd	−1.5	Expressed within MF	Sensory organ precursor cell fate determination; Notch pathway	(Singson et al., 1994)
Bib	−1.5	Required during R8 specification	Lateral inhibition of R8 specification; embryonic neurogenesis	(Rao et al., 1990)
arc	−1.4	Clusters along MF	Adheren junction associated protein required for eye development	(Liu and Lengyel, 2000)
ss	−1.34	MF; expressed in all other imaginal discs	Antennal morphogenesis; transcription factor	(Duncan et al., 1998)
Peripodial Epithelium (12)
Cyp4e2	−3.19	PE of the eye/antennal and wing imaginal discs	Electron transport; steroid metabolism
Osiris 23	−3.16	PE of the eye and leg imaginal discs (Figure 2H)	-
CG11073	−2.59	PE of the eye, leg and wing imaginal discs	-
CG3893	−2.56	PE of the eye imaginal disc	-
Pde8	−2.37	Band just anterior to MF; PE; plasmatocytes; lumen and nuclei of salivary glands; nuclei of fat body	Signal transduction; cyclic nucleotide metabolism; mesoderm development.
beat-Ic	−2.33	Peripodial cell’s nucleoli (Figure 2G)	Axon choice point recognition; cell adhesion; defasciculation
wbl	−1.71	PE of the eye imaginal disc	Exocytosis; protein-Golgi targeting
CG8502	−1.71	Individual cells posterior to MF for 2/3 columns; eye, leg and wing PE; plasmatocytes	Structural constituent of larval cuticle
CG13203	−1.7	PE of the eye imaginal disc; plasmatocytes	-
CG4408	−1.7	PE of the eye imaginal disc	Proteolysis: metallocarboxypeptidase activity
CG2657	−1.5	PE of the eye and wing discs; cells in wing disc	Transport: Glutamate-gated ion channel activity
CG15370	−1.34	PE of the eye imaginal disc	-
Glia (1)
CG31676	−3.22	Retinal basal glial cells (Figure 2F)	-
Plasmatocytes (10)
Pde8	−2.37	Band just anterior to MF; peripodial cells; plasmatocytes; lumen and nuclei of salivary glands; nuclei of fat body	Signal transduction; cyclic nucleotide metabolism; mesoderm dev.
twe	−1.85	Plasmatocytes	Protein tyrosine phosphatase activity; mitosis
CG12508	−1.73	Plasmatocytes	-
CG8502	−1.71	Individual cells posterior to MF for 2/3 columns; eye, leg and wing peripodial cells; plasmatocytes	Structural constituent of larval cuticle
CG13203	−1.7	PE of the eye imaginal disc; plasmatocytes (Figure 2I)	-
CG15911	−1.69	Anterior D/V margins of eye disc;plasmatocytes	-
PGRP-SC2	−1.55	Wing pleurites and plasmatocytes	Peptidoglycan recognition molecule
Smg1	−1.44	Plasmatocytes (Figure 2J)	Nonsense-mediated decay; protein kinase activity
robl62A	−1.44	Plasmatocytes	Microtubule-based movement; Dynein complex; ATPase activity
CG18547	−1.39	Plasmatocytes	Oxidoreductase activity
Other imaginal discs or tissues (5)
Ugt86Di	−3.58	Expressed in the wing disc	-	(Butler et al., 2003)
Obp44a	−1.84	Photoreceptor axons as exiting optic stalk and optic lobe	-
CG8483	−1.63	Expressed in the wing disc	-	(Butler et al., 2003)
PGRP-SC2	−1.55	Wing plurites and plasmatocytes	-
ss	−1.34	MF; Expressed in all other imaginal discs	Antennal morphogenesis; transcription factor	(Duncan et al., 1998)