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. Author manuscript; available in PMC: 2008 Nov 15.
Published in final edited form as: Bioorg Med Chem. 2007 Aug 22;15(22):6927–6936. doi: 10.1016/j.bmc.2007.07.019

Figure 4.

Figure 4

(A) Thermal cleavage assay experiments with polyamide-chlorambucil conjugates (left) 1-Chl and (right) 2-Chl on the on the 280 base pair, 5′ end-labeled PCR product of plasmid pMFST2: lane 1, A reaction; lane 2, G reaction; lanes 3–13, 1 μM, 300 nM, 100 nM, 30 nM, 10 nM, 3 nM, 1 nM, 300 pM, 100 pM, 30 pM, and 10 pM polyamide, respectively; lane 14, intact DNA. (C-D) Thermal cleavage assay experiments with polyamide-chlorambucil conjugates (C, left) 3-Chl and (C, right) 4-Chl and (D, left) 5-Chl and (D, right) 6-Chl on the on the 284 base pair, 5′ end-labeled PCR product of plasmid pMFST: lane 1, A reaction; lane 2, G reaction; lanes 3–13, 100 nM, 30 nM, 10 nM, 3 nM, 1 nM, 300 pM, 100 pM, 30 pM, 10 pM, 3 pM, and 1 pM polyamide, respectively; lane 14, intact DNA. Putative major sites of alkylation on the DNA fragments are indicated by arrows on the sequences adjacent to each gel.