Table 1.

Primers used for mutation analysis, sequencing, cloning and mutagenesis of mouse Bcl11b.

Fragment	Direction	Sequence (5'->3')	Size of PCR product (bp)	Annealing temp. (°C)/Cycles
ex1	F	CGC ATC TGT GCA GCT TTC	226	56/35
	R	CCG GCT GCA GAG AAA CTT
ex2a	F	TGT CTT CTC TGC CCC TTC C	264	59/35
	R	CTT GTC CAG GAC CTT GTC GT
ex2b	F	ACC TGT GGC CAG TGT CAG AT	300	59/35
	R	TAG GGA GGC AGC TTC AGC A
ex3	F	GGC ACC ACT GAC CAG TCT TT	377	61/35
	R	CTT TTT GAG TGG GGG ATG G
zfd1	F	TGC TCA CAC TCT GCC TTT CTT	291	59/35
	R	GCA GCA GGT TGA AAG GAT TG
ex4a	F	TGA ATT TCC TGG GGG ACA G	283	60/35
	R	CTG TTG CCG GCC AGT TCT
prd	F	TCT CCT GCC ATG GAC TTC TC	329	62/35
	R	TGG CAC TTG TAG GGC TTC TC
zfd2	F	AGT CCC AAG TCC CCG TTC	299	60/35
	R	CAG CTG AGA GCC CGT CGT
acd	F	ATG AAG ACG CAC ATG CAC AA	289	64/35
	R	CAC GCC ACC TCC GTT CTC
ex4b	F	AGC ATG GAC TCG GAG CTG	300	62/35
	R	AGC TGG AAA GGG CTC TGC
ex4c	F	GTG ATG CCG GAG CTG TTG	291	59/35
	R	CCA GGA ATG GGT CCT TCA
ex4d	F	GTG TAC TCG CAG TGG CTC GT	294	60/35
	R	GAC CTT GCC GCA GTA CTC AC
zfd3	F	ACA CCT CAC CTG GGT GGT C	274	59/35
	R	TCA TTA GTC AGC AAG TGT TCA CC
cloning	F	TTT GAA TTC ATG TCC CGC CGC AAA C	2459	65/35
	R	TTT GCG GCC GCT TAG CTC CTC TCA GCC TGC TC
S778N	F	CTG GGA GGC CGA ACT CCA AGG AGG GC		65/12
	R	GCC CTC CTT GGA GTT CGG CCT CCC AG
K828T	F	GCA GAG CAG CAC GCT CAC GCG CCA C		65/12
	R	GTG GCG CGT GAG CGT GCT GCT CTG C
Y844C	F	GCA AGG AGG TGT GCC GCT GCG ACA TC		65/12
	R	GAT GTC GCA GCG GCA CAC CTC CTT GC
FS823	F	CGA GCT GTG CAA CTA CGC GCT AAG AGC AGC AAG CTC ACG CG		65/18
	R	CGC GTG AGC TTG CTG CTC TTA GCG CGT AGT TGC ACA GCT CG