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. 2007 Oct 4;8:354. doi: 10.1186/1471-2164-8-354

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Copyright © 2007 Liao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Triple fluorescence in situ hybridizations (ISH) and immunocytochemistry (ICC) for zecac mRNA/zpmca2 mRNA/Na⁺-K⁺-ATPase (A-E) and zecac mRNA/zncx1b mRNA/Na⁺-K⁺-ATPase (F-J) in frozen sections of zebrafish gills. A-E are the same section, and F-J are another section. A, zpmca2 mRNA; B and G, zecac mRNA; C and H, Na⁺-K⁺-ATPase (NKA); D and I, bright field (BF); E, merged image of A-D; J, merged image of F-I. Colocalization of the 3 transporter mRNAs and protein was found respectively in E and J. Scale bar = 20 μm.