Figure 1.

Structure of Mi-1.2. (A) The 14.7-kb genomic clone carrying the Mi-1.2 primary transcript (cross-hatched bar) and flanking sequences (line). This fragment is sufficient to confer resistance to root-knot nematodes to susceptible tomato (7). (B) Mi-1.2 transcript with exons as solid bars and the two introns as angled lines. The arrow marked SM7 represents the approximate position of the primer used to isolate the 5′ ends of transcripts expressed in leaves. (C) Predicted protein structure of Mi-1.2. LZ, the position of a possible leucine zipper; NBS, a nucleotide binding site; LRR, leucine-rich repeat region (8, 9). The NBS-containing region that is highly conserved among many plant resistance-associated proteins is shown in gray.