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DNase I footprints of RNAP bound to the 4192-rrnB P1 promoter. The DNA fragment was labeled at promoter position +50 in the nontemplate strand. Lanes: 1, A+G sequence markers; 2, no RNAP; 3, wild-type (WT) RNAP; 4, αR265A RNAP. Small arrows indicate upstream region positions protected by WT RNAP, but not by αR265A RNAP. Large arrow indicates a position of enhanced DNase I cleavage in the WT RNAP footprint.
