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. 1998 Aug 18;95(17):9779–9784. doi: 10.1073/pnas.95.17.9779

Figure 1.

Figure 1

Interaction of the PDZ domain of AF6 with the C termini of EphB2 and EphB3. (A) AF6-domain topology: ras-binding domain (RBD), homology regions to kinesin-like proteins (U104) and to class V myosins (DIL), PDZ domain (PDZ). cDNAs identified in a two-hybrid screening are shown relative to the enlarged PDZ domain. (B) Intracellular domains of EphB2 and EphB3 containing deletions or point mutations were tested in two-hybrid assays for their ability to bind to the PDZ domain of AF6. Interactions were detected by induction of HIS3 expression on medium lacking histidine. KD refers to the wild-type receptor, KD- to the kinase-deficient receptor. Sequences of C-terminal peptides are indicated. The C-terminal elongation of EphB3 by 15 aa is shown in black.