Figure 2.

Association of EphB3 and the PDZ domain of AF-6 in vitro and in mammalian cells. (A) Wild-type or mutant EphB3 was expressed in COS-1 cells as indicated. Proteins that could be precipitated with the GST-AF6/PDZ fusion (P) were treated on a Western blot (WB) with EphB3 antibodies. (B) Lysates from untransfected, EphB3-transfected, EphB3- and HA-AF6-cotransfected, or HA-AF6-transfected COS-1 cells were immunoprecipitated (IP) with HA antibodies. Immunoprecipitates were probed on a Western blot (WB) with EphB3 antiserum, stripped, and reprobed with monoclonal HA antibodies.