Table 2.

Sequence requirements for the PDZ-mediated interaction of EphB2 and AF6

Mutated EphB2 C terminus	AF6 binding
IQSVEV	+
IQSVEL	−
IQSLEV	+
IQSIEV	+
IQSMEV	−
IQSAEV	−
IQSSEV	+
IQSTEV	+
IQSVPV	+
IQPVPV	−
NGSVPV	−

The C-terminal sequence of wild-type EphB2 (IQSVEV) is given in italics. Single or multiple amino acid substitutions are shown in boldface lettering. LexA peptide fusions were expressed in yeast and their ability to interact with the PDZ domain of AF6 was monitored in two-hybrid assays.