Table 2.

Effect of neomycin on the activity of angiogenin in the CAM assay

Sample	Total eggs	% positive
Angiogenin (10 ng)	76	55
Neomycin (20 ng)	50	20
Neomycin (200 ng)	29	21
Angiogenin (10 ng) + neomycin (4 ng)	40	40
Angiogenin (10 ng) + neomycin (20 ng)	40	20
Angiogenin (10 ng) + neomycin (200 ng)	20	25
Water	128	20

Angiogenesis was measured on the CAM as described (2). Growth of blood vessels was observed microscopically and recorded as either positive or negative after 48 hr of incubation. Data were combined from multiple sets of experiments each using between 10 and 20 eggs. A positive response in ≈20% of the eggs is usually observed in water control and is considered as background angiogenesis which is not affected by inhibitors.