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Spindle pole antigens are similarly localized on poles whether or not centrosomes are present. Microtubules are green, antigens and DNA are red, and overlap is yellow. γ Tubulin (A–C) and NuMA (D–F) immunofluorescence of chromatin bead spindles (A and D) and DMSO asters (B and E) with self-assembled poles and sperm DNA spindles (C and F) with a centrosome at each pole. Bar, 5 μm.
