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. 1997 Aug 11;138(3):615–628. doi: 10.1083/jcb.138.3.615

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Centrosomes are dominant sites of pole assembly. (a) Sperm half spindle reactions in the presence of control antibodies (A and B) or mAb 70.1 (C and D) were evaluated for microtubule polarity by NuMA immunofluorescence. (b) Quantification of monopolar and bipolar structures under each condition. At least 300 spindles were counted for each condition in two separate experiments. Bar, 5 μm.

Centrosomes are dominant sites of pole assembly. (a) Sperm half spindle reactions in the presence of control antibodies (A and B) or mAb 70.1 (C and D) were evaluated for microtubule polarity by NuMA immunofluorescence. (b) Quantification of monopolar and bipolar structures under each condition. At least 300 spindles were counted for each condition in two separate experiments. Bar, 5 μm.