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. 1997 Aug 11;138(3):589–603. doi: 10.1083/jcb.138.3.589

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Temporal expression of the mbc transcript. (A) A Northern blot containing 4 μg of poly A⁺ RNA from several stages of development. Probes included a 1.8-kb genomic EcoRI fragment that includes mbc coding sequence and a 400-bp Xba/ HindIII fragment of α1-tubulin (Theurkauf et al., 1986) as a control for loading. (B) Reverse transcriptase PCR analysis of mbc RNA from unfertilized embryos. 0–4-h embryos, adult females, and adult males as detailed in Materials and Methods.