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Binding of soluble laminin 5 to immobilized type VII procollagen. Microtiter wells were coated with 1 μg of type VII–NC-1 purified from amnion (•) or intact type VII procollagen immunopurified from WISH cell culture medium (□), or type VII procollagen digested with either bacterial collagenase (○) or pepsin (▪). pAb to laminin 5 were used for detecting the amount of bound substrate.
