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Inhibition of type VII–NC-1 binding to immobilized laminin 5 by preincubation with various competitors. The test system consisted of laminin 5 as the immobilized phase at the concentration of 1 μg per well, and a fixed amount of soluble type VII collagen– NC-1 (5 μg/ml). Type VII– NC1 was incubated at 4°C for 8 h with varying concentration of laminin 5 (•) or laminin 1–nidogen complex (○) before testing on the insoluble ligand.
