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. 1997 Aug 11;138(3):719–728. doi: 10.1083/jcb.138.3.719

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Localization of laminin 5 to anchoring plaques. Laminin 5 is localized to anchoring plaques in human skin using mAb BM165 and 5-nm (A, arrow) or 1 nm silver-enhanced colloidal gold (B–D). Labeling of the lamina lucida and anchoring plaques is intense using silver-enhanced 1 nm colloidal gold in comparison to 5 nm colloidal gold. Using a mixture of mAb BM165 (anti-laminin α3) and pAb 3959 (anti-type VII collagen NC-1), laminin 5 is seen to colocalize with type VII collagen in anchoring plaques (E, 1-nm silver-enhanced particles specific for mAb BM165 [small arrow] and 10-nm silver-enhanced particles are specific for pAb 3959 [large arrow]; F, 5-nm particles specific for mAb BM165 (small arrow) and 15-nm particles specific for pAb 3959 [large arrow]. Bar, 100 nm.