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. 1997 Aug 11;138(3):657–669. doi: 10.1083/jcb.138.3.657

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The binding of KIF2 to membrane vesicles. Microsome fraction from developing rat cerebral cortex was fractionated by sucrose gradient centrifugation, and the same volume from each fraction was applied to SDS-PAGE, transferred to PVDF membranes, and analyzed by immunoblotting with antibodies against KHC, KIF2, synaptophysin (p38), synapsin I (Sin I), β_gc, APP, GAP-43, and N-cadherin (N-Cad). Note that the peak fractions of KIF2 and β_gc are highly coincident.