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. 1997 Aug 11;138(3):657–669. doi: 10.1083/jcb.138.3.657

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KIF2 expression in PC12 cells. Immunoblot analysis of whole cell extracts from nontreated (lane 1) or NGF-treated (lane 2) PC12 cells reacted with the BKF2 antibody (dilution 1:50). NGF induces the expression of a single KIF2 immunoreactive protein species with an apparent molecular mass of 97 kD. Note that KIF2 is barely detected in undifferentiated PC12 cells. When NGF-differentiated PC12 cells were deprived of the neurotrophin for 6 h, KIF2 becomes considerably less abundant (lane 3) than in control ones (lane 2). 40 μg of protein were loaded in each lane and the immunoblots were revealed using a rabbit ProtoBlot (Promega) staining kit. PC12 cells were treated with NGF (50 ng/m) for 3 d.