Figure 6.

Effect of the KIF2 antisense oligonucleotide ASKF2a (5 μM) on KIF2 (A), tyrosinated α-tubulin (A), KHC (B), β_gc (C), and synaptophysin (D) protein levels as revealed by Western blot analysis of whole cell homogenates obtained from PC12 cells. For this experiment PC12 cells cultured in the presence of NGF were treated for 24 h with the ASKF2a oligonucleotide. Antisense treatment was initiated 3 d after the addition of NGF (50 ng/ml). Control cultures were treated with equivalent doses of sense oligonucleotides. (A) Blot reacted with antibodies against KIF2 (RKF2 diluted 1:50) and tyrosinated α-tubulin (mAb TUB-1A2), or with mAb SUK 4 (B), or with an affinity-purified polyclonal antibody against β_gc (C), or with mAb SY38 (D). 30 μg of total cellular protein were loaded in each lane. Blots were revealed using the ProtoBlot staining kit (Promega).