Skip to main content
. 1997 Aug 11;138(3):629–641. doi: 10.1083/jcb.138.3.629

Figure 2.

Figure 2

GFP–TUB3 expression and incorporation into functional microtubules. (A) Western blot of crude yeast protein extracts probed with anti–α-tubulin antibody. Strain TSY425, containing the GFP–TUB3 plasmid, and strain TPS510, containing the vector plasmid, were grown in the presence of either 2% galactose, or 2% galactose + 0.5% glucose as the carbon source. Both strains show endogenous total α-tubulin (TUB1p, TUB3p) running as a doublet at 56 kD, while the GFP–TUB3 fusion protein runs at 83 kD. Similar results were obtained with a COOH-terminal fusion protein, TUB3–GFP (data not shown). (B) GFP– TUB3 expression rescues the benomyl supersensitivity of a tub3:: TRP1 haploid strain (DBY2375). (Top) Growth of tub3::TRP1 cells transformed with plasmids as noted above, and a 1:10 dilution of cells (second row), when grown with 2% galactose. (Bottom) Growth of the same strains, and 1:10 dilutions, in the presence of 5 μg/ml benomyl. All cells were incubated at 30°C. tub3:: TRP1 containing either vector alone, TUB3–GFP, or TUB1–GFP remain benomyl supersensitive, while both TUB3 and GFP– TUB3 rescue the benomyl supersensitivity.