Table II.
Microtubule Interactions with the Cell Cortex
| Parameter | Wild type | Dynein mutant | ||
|---|---|---|---|---|
| Percentage of time at cortex (n = MTs) | 42.7 (n = 39) | 83.0 (n = 36) | ||
| Unbudded* | 44.5 (n = 24) | 92.8 (n = 10) | ||
| Budded | 41.1 (n = 15) | 79.3 (n = 26) | ||
| Maximum length of MT (μm) | 2.0 | 4.0 | ||
| Unbudded | 1.9 | 2.3 | ||
| Budded | 2.0 | 4.6 | ||
| Total cells analyzed | 52 | 57 | ||
| Unbudded | 40 | 24 | ||
| Budded | 12 | 33 | ||
| Total cortex interactions (%) | 84.6 | 77.2 | ||
| Unbudded | 85.0 | 79.2 | ||
| Budded | 83.3 | 75.8 | ||
| Hit cortex (%) | 71.2 | 68.4 | ||
| Unbudded | 72.5 | 70.8 | ||
| Budded | 66.7 | 66.7 | ||
| Grow at cortex (%) | 32.7 | 19.3 | ||
| Unbudded | 35.0 | 16.7 | ||
| Budded | 25.0 | 21.2 | ||
| Sweep on cortex (%) | 32.7 | 33.3 | ||
| Unbudded | 25.0 | 33.3 | ||
| Budded | 58.3 | 33.3 | ||
| Shrink at cortex (%) | 5.8 | − | ||
| Unbudded | 2.5 | − | ||
| Budded | 16.7 | − | ||
| Total nondynamic cells (%) | 1.9 | 14.0 | ||
| Unbudded | − | 12.5 | ||
| Budded | 8.3 | 15.2 |
*
Data from unbudded cells and cells containing a very small bud but no discernible spindle were combined for this and all subsequent measurements.