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. 1997 Aug 11;138(3):485–494. doi: 10.1083/jcb.138.3.485

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Nuclear distribution defects in Class III-mdm1 cells. MYY290 (MDM1), Class IIIa mutants MYY702 (mdm1-200) and MYY710 (mdm1-217), and Class IIIb mutants MYY715 (mdm1-227) and MYY717 (mdm1-228) were synchronized with α-factor at 23°C, released from the cell cycle block at 37°C, fixed with formaldehyde after 0, 2, or 4 h, and stained with DAPI. For each sample, the distribution of phenotypes among 300 cells was determined. Values represent the mean ± standard deviation for triplicate samples. Between 85 and 95% of cells in 0 h samples were unbudded. Inset shows symbols corresponding to each culture, and each phenotype is represented below the x axis. (A) 2 h after release at 37°C. (B) 4 h after release at 37°C.