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. 1997 Aug 11;138(3):643–656. doi: 10.1083/jcb.138.3.643

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Northern blot analysis of IAK1 mRNA expression in adult mouse tissues. Total RNA (30 μg) from adult mouse tissues was separated on a formaldehyde-agarose gel and blotted onto nytran membranes. The blot was hybridized with an 850-bp fragment derived from the PCR clone. The blot was stripped and re-probed with GAPDH to quantitate RNA loading.