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. 1997 Aug 11;138(3):643–656. doi: 10.1083/jcb.138.3.643

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Immunocytochemical localization of IAK1 during the cell cycle. Dividing cultures of NIH 3T3 cells were fixed in ice-cold methanol and then triple-stained with DAPI for DNA, with an anti–β-tubulin monoclonal antibody for microtubules and with an anti-IAK1 antiserum. The cells were visualized with a laser scanning confocal microscope as described in Materials and Methods. Cells are at the following stages of the cell cycle: interphase (A), prophase (B), prometaphase (C), metaphase (D), late anaphase (E), telophase (F), and cytokinesis (G). Bar, 5 μm.