Table II.
Plasmids Used in This Study
| Plasmid | Description | Source | ||
|---|---|---|---|---|
| pLG1 | GAL1-VPH1 in CEN:URA3 plasmid, pCJR52 | This study | ||
| pLG39 | Integrating GAL1-VPH1 construct: GAL1 promoter fused to νph1-Δ369 allele in pRS306 | This study | ||
| pRCP131 | GAL1 promoter fused to PHO8 ORF in CEN-URA3 plasmid | This study | ||
| pRCP132 | GAL1-PHO8 integrating plasmid: GAL1 promoter fused to pho8-Δ329 allele in pRS305 | This study | ||
| pRCP39 | GAL1-PEP4 in CEN-URA3 plasmid | This study | ||
| pRCP95 | νps10-10* LEU2 integrating plasmid (VPS10 without cytosolic tail; STOP codon at amino acid 1,425) | This study | ||
| pLC4-8 | sec4-ts integrating plasmid | Nothwehr et al., 1995 | ||
| pSN92 | PHO8 in CEN-URA3 plasmid | Nothwehr et al., 1993 | ||
| pNB3 | PHO8 lacking codons 2–21 in CEN-URA3 plasmid | This study | ||
| pNB4 | PHO8 lacking codons 2–31 in CEN-URA3 plasmid | This study | ||
| pCC1 | VAM3 gene in pRS316 | This study | ||
| pRCP160 | GST-VAM3 fusion construct | This study | ||
| pRCP161 | VAM3-TMΔ allele. GAL1 promoter fused to fragment encoding cytosolic domain of Vam3p | |||
| pAIN1 | ALP/DPAP B fusion construct: codons 1–31 of PHO8 fused to codons 29–842 of DAP2 behind the GAL1 promoter in CEN-URA3 plasmid | This study | ||
| pAIN2 | Δ11 ALP/DPAP B fusion construct: codons 1, 12–31 of PHO8 fused to codons 29–842 of DAP2 behind the GAL1 promoter in CEN-URA3 plasmid | This study | ||
| pSN100 | (F85,F87→ A,A) A-ALP in CEN-URA3 plasmid | Nothwehr et al., 1993 | ||
| pCJR6 | GAL1-DAP2 construct in CEN:URA3 plasmid | Roberts et al., 1989 | ||
| pRCP56 | νps45-ts (νps45-13) integrating plasmid | Piper et al., 1994 | ||
| pKJH2 | νps27Δ::LEU2 disruption plasmid | Raymond et al., 1992 | ||
| pRCP41 | νps45Δ disruption plasmid (loop in/loop out) | Piper et al., 1994 | ||
| pRCP134 | νps45Δ::URA3 disruption plasmid | This study | ||
| pSN111 | pho8-Δ X disruption plasmid (loop in/loop out) | Nothwehr et al., 1995 | ||
| pCJR18 | dap2Δ::LEU2 disruption plasmid | Roberts et al., 1989 |