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. 1997 Aug 11;138(3):531–545. doi: 10.1083/jcb.138.3.531

Table II.

Plasmids Used in This Study

Plasmid Description Source
pLG1 GAL1-VPH1 in CEN:URA3 plasmid, pCJR52 This study
pLG39 Integrating GAL1-VPH1 construct: GAL1 promoter fused to νph1-Δ369 allele in pRS306 This study
pRCP131 GAL1 promoter fused to PHO8 ORF in CEN-URA3 plasmid This study
pRCP132 GAL1-PHO8 integrating plasmid: GAL1 promoter fused to pho8-Δ329 allele in pRS305 This study
pRCP39 GAL1-PEP4 in CEN-URA3 plasmid This study
pRCP95 νps10-10* LEU2 integrating plasmid (VPS10 without cytosolic tail; STOP codon at amino acid 1,425) This study
pLC4-8 sec4-ts integrating plasmid Nothwehr et al., 1995
pSN92 PHO8 in CEN-URA3 plasmid Nothwehr et al., 1993
pNB3 PHO8 lacking codons 2–21 in CEN-URA3 plasmid This study
pNB4 PHO8 lacking codons 2–31 in CEN-URA3 plasmid This study
pCC1 VAM3 gene in pRS316 This study
pRCP160 GST-VAM3 fusion construct This study
pRCP161 VAM3-TMΔ allele. GAL1 promoter fused to fragment encoding cytosolic domain of Vam3p
pAIN1 ALP/DPAP B fusion construct: codons 1–31 of PHO8 fused to codons 29–842 of DAP2 behind the GAL1  promoter in CEN-URA3 plasmid This study
pAIN2 Δ11 ALP/DPAP B fusion construct: codons 1, 12–31 of PHO8 fused to codons 29–842 of DAP2 behind the GAL1  promoter in CEN-URA3 plasmid This study
pSN100 (F85,F87→ A,A) A-ALP in CEN-URA3 plasmid Nothwehr et al., 1993
pCJR6 GAL1-DAP2 construct in CEN:URA3 plasmid Roberts et al., 1989
pRCP56 νps45-ts (νps45-13) integrating plasmid Piper et al., 1994
pKJH2 νps27Δ::LEU2 disruption plasmid Raymond et al., 1992
pRCP41 νps45Δ disruption plasmid (loop in/loop out) Piper et al., 1994
pRCP134 νps45Δ::URA3 disruption plasmid This study
pSN111 pho8-Δ X disruption plasmid (loop in/loop out) Nothwehr et al., 1995
pCJR18 dap2Δ::LEU2 disruption plasmid Roberts et al., 1989