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. 1997 Aug 11;138(3):707–717. doi: 10.1083/jcb.138.3.707

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Interference with the activity of TSP-1 and its antiangiogenic peptides by soluble GST–CD36 fusion proteins. Increasing concentrations of CD36 fusion proteins that contain a TSP-1 binding site, FP93-120 (circles) or FP93-298 (triangles), or a CD36 fusion protein that lacks a TSP-1 binding site, FP298-439 (squares), were preincubated for 2 h at 4°C with (A) 2 nM TSP-1, (B) 10 μM Mal III peptide, (C) 30 μM Col overlap peptide, or (D) control media. Each mixture was then tested for the ability to block bovine capillary endothelial cell migration towards bFGF (solid symbols) or influence background migration in the absence of bFGF (D, open symbols). Data, accumulated from nine experiments, are reported as a percentage of maximum migration, where 100% represents the number of cells migrating towards the inducer bFGF alone, and 0% corresponds to the number of cells migrating randomly in the absence of inducer (Bkgd). 100% varied between experiments from 32 to 81 cells migrated/10 high-power fields. Bars indicate standard errors.

Interference with the activity of TSP-1 and its antiangiogenic peptides by soluble GST–CD36 fusion proteins. Increasing concentrations of CD36 fusion proteins that contain a TSP-1 binding site, FP93-120 (circles) or FP93-298 (triangles), or a CD36 fusion protein that lacks a TSP-1 binding site, FP298-439 (squares), were preincubated for 2 h at 4°C with (A) 2 nM TSP-1, (B) 10 μM Mal III peptide, (C) 30 μM Col overlap peptide, or (D) control media. Each mixture was then tested for the ability to block bovine capillary endothelial cell migration towards bFGF (solid symbols) or influence background migration in the absence of bFGF (D, open symbols). Data, accumulated from nine experiments, are reported as a percentage of maximum migration, where 100% represents the number of cells migrating towards the inducer bFGF alone, and 0% corresponds to the number of cells migrating randomly in the absence of inducer (Bkgd). 100% varied between experiments from 32 to 81 cells migrated/10 high-power fields. Bars indicate standard errors.