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. 1997 Aug 11;138(3):671–680. doi: 10.1083/jcb.138.3.671

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Intracellular APPβ is observed only in NT2N neurons. Samples of cell lysate and medium collected from cultures of NT2N, NT2, M17, and CHO cells stably expressing APP695 (CHO695) were processed for immunoprecipitation with the antibody Karen. The immunoprecipitates were separated by SDS-PAGE gels and transferred onto nitrocellulose replicas. APPβ present in the cell lysates and the media were detected by immunoblotting with the anti-APPβ antibody 53 (A). After stripping the nitrocellulose replica in A with 0.1% SDS, the blot was reprobed with Karen to detect all APP ectodomain species (B).