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. 1997 Aug 11;138(3):671–680. doi: 10.1083/jcb.138.3.671

Figure 7.

Figure 7

Intracellular β and γ cleavages occur in a pre-Golgi compartment in NT2N neurons. Cultures of NT2N cells were first preincubated with 20 μg/ml BFA for 1 h before radiolabeling with [35S]methionine for 16 h in the continuous presence of 20 μg/ml BFA. Control cultures were processed similarly, except that BFA was absent in the medium. Radiolabeled proteins from BFA-treated and untreated cell lysates and media were immunoprecipitated with Karen (for APPFL in the cell lysates and APPα/β in the media as shown in A), with antibody 53 (for APPβ in B), and with the mAb 6E10 (for Aβ in C). Note that APPβ and Aβ were recovered in the cell lysate but not in the medium of BFA-treated cells. (M, mature APPFL; I, immature APPFL.