Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Aug 11;138(3):671–680. doi: 10.1083/jcb.138.3.671

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

APPβ generated in the presence of BFA is partially glycosylated. Cultures of NT2N neurons were metabolically labeled as in Fig. 7 in the presence or absence of 20 μg/ml BFA. The cell lysates were then immunoprecipitated with the antibody 53. (A) Samples in lanes 3 and 4 were treated with Nglyc F for 16 h to remove N-linked sugars, whereas immunoprecipitates in lanes 1 and 2 were treated with the vehicle. (B) Samples in lanes 2 and 4 were deglycosylated with a combination of Nglyc F, neuraminidase, and O-glycosidase for 16 h to remove both N- and O-linked chains (lanes 2 and 4); lanes 1 and 3 represent samples that were mock digested.