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. 1997 Aug 11;138(3):575–588. doi: 10.1083/jcb.138.3.575

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Actin binding to three abLIM isoforms expressed in vitro. Polypeptides expressed in vitro using constructs corresponding to abLIM-l, abLIM-m, and abLIM-s were tested for their ability to bind to actin filaments. All three isoforms cosediment with F-actin (+actin) and are found primarily in the respective pellet fractions (P). Each isoform is found in the supernatant fraction (S) when actin is not present in the assay mixture (−actin). Nonspecific binding to the GST-only polypeptide was negligible at all concentrations (data not shown). Approximate M _r (kD) of each isoform is indicated at left.